Journal: Cellular & Molecular Biology Letters

Article Title: New mechanistic understanding of osteoclast differentiation and bone resorption mediated by P2X7 receptors and PI3K-Akt-GSK3β signaling

doi: 10.1186/s11658-024-00614-5

Figure Lengend Snippet: The regulatory effect of P2X7 receptor via the PI3K/AKT/GSK3β signaling pathway in vivo. A Western blot analysis measured the phosphorylation levels and grayscale values of P13K, AKT, and GSK3β proteins in mouse femur tissue from each group; B immunohistochemistry staining assessed the expression and positive area percentage of p-P13K, p-AKT, and p-GSK3β in mouse femur tissue of each group; C bar graph presenting the number of TRAP-positive cells in mouse femur tissue sections stained with TRAP; D western blot analysis determined the expression levels and band grayscale values of MMP9, NFATc1, and CK proteins in mouse femur tissue from each group; E immunohistochemistry staining assessed the expression and positive area percentage of MMP9 and NFATc1 in mouse femur tissue; F ELISA measured the expression levels of CTX and NTX in mouse serum; G Von Kossa staining assessed the calcium salt deposition in mouse femur tissue sections. Bar = 100 μm. Compared with the WT + Sham group, *** P < 0.001; compared with the WT + OVX group, ### P < 0.001; compared with the KO + OVX group, &&& P < 0.001

Article Snippet: We used a mouse Cross-linked Type I Collagen C-Telopeptide (CTX) ELISA kit (M3023, Elabscience, Wuhan, China) and a mouse Cross-linked Type I Collagen N-Telopeptide (NTX) ELISA kit (E-EL-M3022, Elabscience, Wuhan, China) for mice.

Techniques: In Vivo, Western Blot, Phospho-proteomics, Immunohistochemistry, Staining, Expressing, Enzyme-linked Immunosorbent Assay